![]() ![]() J Mol Biol 215:403–410īreatnach R, Chambon P (1981) Organization and expression of eucaryotic split genes coding for proteins. sylvestris has become an endangered species in Belgium, the knowledge gathered in this study will be an important tool for selecting useful genotypes for a core collection.Īltschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. sylvestris will enable their introduction into new breeding strategies. The determination of the S-RNase genotypes of old cultivars and M. It provided some clues to the occurrence of new S-alleles among the multitude of novel S-RNase sequences that have been identified, which were mostly unique for the group of M. Based on the sequence of all different SCAR-fragments, which comprise both the hypervariable PS1 region and the single intron, S-RNase genetic diversity was further explored. sylvestris as well, which points to strong conservation of the S-locus gene structure. The majority of the M. × domestica S-alleles has been found in M. Heterogeneity of the S-RNase allelic distribution is much higher in cultivated apple than in wild apple, which shows that breeding leads to strong departure from the expected homogeneity of genes under negative frequency-dependent selection. ![]() sylvestris was much higher than in M. × domestica, indicating the negative influence of domestication on S-RNase diversity. sylvestris- M. × domestica hybrids was determined. Using PCR-based approaches, the S-RNase genotype of 140 M. × domestica cultivars, 196 M. ![]() Malus S-RNase genetic diversity was analyzed in Malus × domestica cultivars and compared to European wild apple ( Malus sylvestris). ![]()
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